Part:BBa_K4211003:Design

LL-37 secretion system

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

• No BioBrick restriction enzyme cut sites in coding region.
• Constructed by PCR.

Forward primer

5'- CGC GGA TCC ATG TAC AGG ATG CAA CTC CTG T -3'

Reverse primer

5'- TTT ATA GCG GCC GCT TAG GAC TCT GTC CTG GGT ACA AG -3'

Source

The BNUZH-China iGEM team 2022 designed and built.

References

[1] Wang, G. et al. Design of Antimicrobial Peptides: Progress Made with Human Cathelicidin LL-37. Adv Exp Med Biol 1117, 215-240 (2019). https://doi.org:10.1007/978-981-13-3588-4_12
[2] Stecher, C., Maurer, K. P., Kastner, M. T. & Steininger, C. Human Cytomegalovirus Induces Vitamin-D Resistance In Vitro by Dysregulating the Transcriptional Repressor Snail. Viruses 14 (2022). https://doi.org:10.3390/v14092004
[3] Chessa, C. et al. Antiviral Effect of hBD-3 and LL-37 during Human Primary Keratinocyte Infection with West Nile Virus. Viruses 14 (2022). https://doi.org:10.3390/v14071552